癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
SCUBE3 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| E, IHC-P |
|---|---|
| Primary Accession | Q8IX30 |
| Other Accession | NP_689966, 31377568 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 109282 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | SCUBE3 antibody can be used for detection of SCUBE3 by immunohistochemistry at 5 µg/mL. |
| Gene ID | 222663 |
|---|---|
| Other Names | Signal peptide, CUB and EGF-like domain-containing protein 3, SCUBE3 (HGNC:13655) |
| Target/Specificity | SCUBE3; |
| Reconstitution & Storage | SCUBE3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | SCUBE3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SCUBE3 (HGNC:13655) |
|---|---|
| Function | Is a positive regulator of the BMP signaling pathway, required for proper chondrogenesis, osteogenesis and skeletal development. It acts as a coreceptor for BMP ligands, particularly BMP2 and BMP4, facilitating their interactions with BMP type I receptors (PubMed:33308444). It is required for ligand-induced recruitment of BMP receptors to lipid rafts (By similarity). Binds to TGFBR2 and activates TGFB signaling. In lung cancer cells, could serve as an endogenous autocrine and paracrine ligand of TGFBR2, which could regulate TGFBR2 signaling and hence modulate epithelial-mesenchymal transition and cancer progression. |
| Cellular Location | Secreted. Cell surface |
| Tissue Location | Highly expressed in osteoblasts. In normal lung, mainly expressed in bronchial epithelial cells. Tends to be up- regulated in lung cancer cells. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
SCUBE3 Antibody: SCUBE3 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and is highly expressed in primary osteoblasts and bones, and to a lesser extent in heart (1,2). Other studies have shown that overexpression of SCUBE3 in mice induced cardiac hypertrophy, suggesting that it may also play a role in the regulation of cardiac growth. SCUBE3 has been shown to be an endogenous TGF-beta receptor ligand (3,4) and is thought to promote lung cancer cell mobility and invasiveness. In lung cancer cells, the secreted SCUBE3 protein was cleaved by MMP2 and MMP9, allowing the activation of the TGF-beta receptor, the increase of Smad2/3 transcriptional activity and the upregulation of expression of proteins such as TGF-beta1, VEGF, Snail, and Slug.
REFERENCES
Wu BT, Su YH, Tsai MT, et al. A novel secreted, cell-surface glycoprotein containing multiple epidermal growth factor-like repeats and one CUB domain is highly expressed in primary osteoblasts and bones. J. Biol. Chem. 2004; 279:37485-90.
Yang RB, Ng CK, Wasserman SM, et al. Identification of a novel family of cell-surface proteins expressed in human vascular endothelium. J. Biol. Chem. 2002; 277:46364-73.
Yang HY, Cheng CF, Djoko B, et al. Transgenic overexpression of the secreted, extracellular EGF-CUB domain containing protein SCUBE3 induces cardiac hypertrophy in mice. Cardiovas. Res. 2007; 75:139-47.
Wu YY, Peck K, Chang YL, et al. SCUBE3 is an endogenous TGF-β receptor ligand and regulates the epithelial-mesenchymal transition in lung cancer. Oncogene 2011; 30:3682-93.
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