癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
ZHANGFEI Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, ICC, E |
|---|---|
| Primary Accession | Q9NS37 |
| Other Accession | NP_001034707, 88900495 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 37134 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | ZHANGFEI antibody can be used for detection of ZHANGFEI by Western blot at 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 10 µg/mL. |
| Gene ID | 58487 |
|---|---|
| Other Names | CREB/ATF bZIP transcription factor, Host cell factor-binding transcription factor Zhangfei, HCF-binding transcription factor Zhangfei, CREBZF, ZF |
| Target/Specificity | CREBZF; |
| Reconstitution & Storage | ZHANGFEI antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | ZHANGFEI Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CREBZF |
|---|---|
| Synonyms | ZF |
| Function | Strongly activates transcription when bound to HCFC1. Suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1-dependent manner. Also suppresses the HCFC1-dependent transcriptional activation by CREB3 and reduces the amount of CREB3 in the cell. Able to down-regulate expression of some cellular genes in CREBZF-expressing cells. |
| Cellular Location | Nucleus. Note=Colocalizes in promyelocytic leukemia protein nuclear bodies (PML- NB) with CREB3 and HCFC1 |
| Tissue Location | In adults, expressed most abundantly in heart, liver and skeletal muscle, moderately abundant in kidney and pancreas, and barely detectable in lung. In fetal tissues, expressed most abundantly in kidney and very low amounts in heart, lung and liver |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
ZHANGFEI Antibody: ZHANGFEI, also known as ZF, is a basic region-leucine zipper transcriptional factor that has been implicated in herpes virus infection cycle and related cellular processes. ZHANGFEI protein has a negatively charged active N-terminus, a leucine zipper of six heptad leucine repeats separated by a conserved six amino acid spacer, a basic domain and a bZIP region. ZHANGFEI strongly activates transcription and suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1 dependent manner. It also suppresses the HCFC1 dependent transcriptional activation by CREB3 and reduces the amount of CREB3 in the cell.
REFERENCES
Hogan MR, Cockram GP and Lu R. Cooperative interaction of Zhangfei and ATF4 in transactivation of the cyclic AMP response element. FEBS Lett. 2006; 580:58-62.
Cockram GP, Hogan MR, Burnett HF, et al. Identification and characterization of the DNA-binding properties of a Zhangfei homologue in Japanese pufferfish, Takifugu rubripes. Biochem. Biophys. Res. Commun. 2005; 339:1238-45.
Lu R and Misra V. Zhangfei: a second cellular protein interacts with herpes simplex virus accessory factor HCF in a manner similar to Luman and VP16. Nucleic Acids Res. 2000; 28:2446-54.
Misra V, Rapin N, Akhova O, et al. 2005. Zhangfei is a potent and specific inhibitor of the host cell factor-binding transcription factor Luman. J. Biol. Chem. 2005; 280:15257-66.
终于等到您。ABCEPTA（百远生物）抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息，您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。
如有疑问，联系：0512-88856768 tech-china@abcepta.com.
