癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
CMPK2 Antibody
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- 背景知识
Application 
| WB, IF, E |
|---|---|
| Primary Accession | Q5EBM0 |
| Other Accession | NP_997198, 117606370 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 49448 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | CMPK2 antibody can be used for detection of CMPK2 by Western blot at 1 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 129607 |
|---|---|
| Other Names | UMP-CMP kinase 2, mitochondrial, 2.7.4.14, Nucleoside-diphosphate kinase, 2.7.4.6, CMPK2 |
| Target/Specificity | CMPK2; Multiple isoforms of CMPK2 are known to exist. |
| Reconstitution & Storage | CMPK2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | CMPK2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | CMPK2 |
|---|---|
| Function | Mitochondrial nucleotide monophosphate kinase needed for salvage dNTP synthesis that mediates immunomodulatory and antiviral activities through IFN-dependent and IFN-independent pathways (PubMed:17999954, PubMed:30083606, PubMed:36930652, PubMed:37075076). Restricts the replication of multiple viruses including flaviviruses or coronaviruses (PubMed:30083606, PubMed:36930652, PubMed:37075076). Together with viperin/RSAD2 and ddhCTP, suppresses the replication of several coronaviruses through inhibition of the viral RNA-dependent RNA polymerase activities (PubMed:36930652). Concerning flaviviruses, restricts RNA translation when localized to the mitochondria independently of its kinase activity (PubMed:37075076). Is able to phosphorylate dUMP, dCMP, CMP, UMP and monophosphates of the pyrimidine nucleoside analogs ddC, dFdC, araC, BVDU and FdUrd with ATP as phosphate donor. Efficacy is highest for dUMP followed by dCMP while CMP and UMP are poor substrates. Controls therefore mitochondrial DNA synthesis by supplying required deoxyribonucleotides (By similarity). CMPK2-dependent mitochondrial DNA synthesis is necessary for the production of oxidized mitochondrial DNA fragments after exposure to NLRP3 activators (By similarity). In turn, cytosolic oxidized mtDNA associates with the NLRP3 inflammasome complex and is required for its activation (By similarity). |
| Cellular Location | Mitochondrion. Note=Mitochondrial localization is required for its antiviral function. |
| Tissue Location | High levels are observed in myeloid, lymphoid and mesenchymal tissues. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
CMPK2 Antibody: UMP-CMP kinase 2 (CMPK2) is the first nucleoside monophosphate kinase that has been identified in human mitochondria. It is a component of the salvage pathway for nucleotide synthesis that may participate in terminal differentiation of monocytic cells. There are two distinct domains of CMPK2: the N-terminal domain with unknown function and the C-terminal domain with the kinase function, suggesting that CMPK2 may be a bifunctional protein with other biological functions in addition to its UMP-CMP kinase activity. CMPK2 may participate in dUTP and dCTP synthesis and is responsible for phosphorylation of dCMP and dUMP in mitochondria. CMPK2 is actively involved in macrophage activation and the inflammatory response
REFERENCES
Xu Y, Johansson M, Karlsson A. Human UMP-CMP kinase 2, a novel nucleoside monophosphate kinase localized in mitochondria. J. Biol. Chem. 2008; 283:1563-71.
Chen YL, Lin DW, and Chang ZF. Identification of a putative human mitochondrial thymidine monophosphate kinase associated with monocytic/macrophage terminal differentiation. Genes Cells 2008; 13:679-89.
Arima T, Akiyoshi H, and Fujii S. Characterization of pyrimidine nucleoside monophosphokinase in normal and malignant tissues. Cancer Res. 1977; 37:1593-7.
Goertsches RH, Hecker M, Koczan D, et al. Long-term genome-wide blood RNA expression profiles yield novel molecular response candidates for IFN-beta-1b treatment in relapsing remitting MS. Pharmacogenomics 2010;11:147-61.
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