癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
KDM1B Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | Q8NB78 |
| Other Accession | NP_694587, 116256451 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 92098 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | KDM1B antibody can be used for detection of KDM1B by Western blot at 1 - 2 µg/ml. |
| Gene ID | 221656 |
|---|---|
| Other Names | Lysine-specific histone demethylase 1B, 1.-.-.-, Flavin-containing amine oxidase domain-containing protein 1, Lysine-specific histone demethylase 2, KDM1B, AOF1, C6orf193, LSD2 |
| Target/Specificity | KDM1B; KDM1B antibody is human, mouse and rat reactive. At least two isoforms of KDM1B are known to exist; this KDM1B antibody will detect both isoforms. |
| Reconstitution & Storage | KDM1B antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | KDM1B Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | KDM1B (HGNC:21577) |
|---|---|
| Function | Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of GLYR1 to achieve such activity, they form a multifunctional enzyme complex that modifies transcribed chromatin and facilitates Pol II transcription through nucleosomes (PubMed:30970244). |
| Cellular Location | Nucleus. Chromosome. Note=Found in actively RNAPolII- transcribed gene bodies. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Flavin-dependent histone demethylases regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (reviewed in 1). KDM1B is a recently identified histone H3K4 demethylase that is required to establish maternal genomic imprints; targeted disruption of the KDM1B gene in mice led to lethality in their embryos, suggesting demethylation of H3K4 is critical for establishing the DNA methylation imprints (2). KDM1B has also been shown to play a role in the transcription elongation in several genes (3).
REFERENCES
Culhane JC and Cole PA. LSD1 and the chemistry of histone demethylation. Curr. Opin. Chem. Biol. 2007; 11:561-8.
Ciccone DN, Su H, Hevi S, et al. KDM1B is a histone H3K4 demethylase required to establish maternal genomic imprints. Nature 2009; 461:415-8.
Fang R, Barbera A, Xu Y, et al. Human LSD2/KDM1b/AOF1 regulates genes transcription by modulating intragenic H3K4me2 methylation. Mol. Cell 2010; 39:222-233.
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