癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
GRK6 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | P43250 |
| Other Accession | NP_001004106, 51896039 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 65991 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | GRK6 antibody can be used for detection of GRK6 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 2870 |
|---|---|
| Other Names | G protein-coupled receptor kinase 6, 2.7.11.16, G protein-coupled receptor kinase GRK6, GRK6, GPRK6 |
| Target/Specificity | GRK6; GRK6 antibody is human, mouse and rat reactive. Multiple isoforms of GRK6 are known to exist. This antibody is predicted to not cross-react with other members of the GRK protein family. |
| Reconstitution & Storage | GRK6 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | GRK6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | GRK6 |
|---|---|
| Synonyms | GPRK6 |
| Function | Specifically phosphorylates the activated forms of G protein- coupled receptors (PubMed:19423652, PubMed:20048153). Such receptor phosphorylation initiates beta-arrestin-mediated receptor internalization, and signaling events leading to their desensitization (PubMed:19423652, PubMed:20048153). Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (PubMed:20048153). Phosphorylates rhodopsin (RHO) (in vitro) and a non G protein-coupled receptor: LRP6 during Wnt signaling (in vitro) (PubMed:19801552). |
| Cellular Location | Membrane; Lipid-anchor. |
| Tissue Location | Widely expressed.. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The G protein–coupled receptor kinases (GRKs) are a versatile family of kinases that play a critical role in cancer metastasis through their regulation of G-protein coupled receptors (GPCRs) involved in growth factor mediated cell migration (1,2). Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state (1). GRK6 is one of 7 members of the GRK serine/threonine kinase subfamily, which has been shown to modulate the Wnt signaling pathway via phosphorylation of LRP6 (3,4), and the insulin-like growth factor signaling pathway (4). GRK6 may also play a role in immune system function (5).
REFERENCES
Inglese J, Freedman NJ, Koch WJ, et al. Structure and mechanism of the G protein-coupled receptor kinases. J. Biol. Chem. 1993; 268:23735-8.
Raghuwanshi SK, Smith N, Rivers EJ, et al. G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis. J. Immunol. 2013; 190:5329-36.
Benovic JL and Gomez J. Molecular cloning and expression of GRK6. A new member of the G protein-coupled receptor kinase family. J. Biol. Chem. 1993; 268:19521-7.
Chen M, Philipp M, Wang J, et al. G Protein-coupled receptor kinases phosphorylate LRP6 in the Wnt pathway. J. Biol. Chem. 2009; 284:35040-8.
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