癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
POU2F3 Antibody
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- 实验流程
- 背景知识
Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | Q9UKI9 |
| Other Accession | NP_001231611, 347658964 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 47432 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | POU2F3 antibody can be used for detection of POU2F3 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL. |
| Gene ID | 25833 |
|---|---|
| Other Names | POU domain, class 2, transcription factor 3, Octamer-binding protein 11, Oct-11, Octamer-binding transcription factor 11, OTF-11, Transcription factor PLA-1, Transcription factor Skn-1, POU2F3, OTF11, PLA1 |
| Target/Specificity | POU2F3; POU2F3 antibody is human, mouse and rat reactive. At least two isoforms are known to exist. This antibody will recognize both isoforms. POU2F3 antibody is predicted to not cross-react with other members of the POU domain class 2 family. |
| Reconstitution & Storage | POU2F3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | POU2F3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | POU2F3 (HGNC:19864) |
|---|---|
| Synonyms | OTF11, PLA1 |
| Function | Transcription factor that binds to the octamer motif (5'- ATTTGCAT-3') and regulates cell type-specific differentiation pathways. Involved in the regulation of keratinocytes differentiation (PubMed:11329378). The POU2F3-POU2AF2/POU2AF3 complex drives the expression of tuft-cell-specific genes, a rare chemosensory cells that coordinate immune and neural functions within mucosal epithelial tissues (PubMed:35576971). |
| Cellular Location | Nucleus {ECO:0000250|UniProtKB:P31362}. |
| Tissue Location | Specifically expressed in epidermis and cultured keratinocytes. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
POU2F3, also known as Epoc-1, is a member of a family of POU domain family of transcription factors (1). POU2F3 is expressed primarily in the epidermis and plays a critical role in keratinocyte proliferation and differentiation (1,2). It is a crucial transcription factor that is required for the development of sweet, umami, and bitter, but not sour taste receptor cells (3). POU2F3 is also a candidate tumor suppressor protein, and aberrant promoter methylation of this gene may play a role in cervical cancer (4).
REFERENCES
Yukawa K, Yasui T, Yamamoto A, et al. Epoc-1: a POU-domain gene expressed in murine epidermal basal cells and thymic stromal cells. Gene 1993; 133:163-9.
Cabral A, Fischer DF, Vermeij WP, et al. Distinct functional interactions of human Skn-1 isoforms with Ese-1 during keratinocyte terminal differentiation. J. Biol. Chem. 2003; 278:17792-9.
Matsumoto I, Ohmoto M, Narukawa M, et al. Skn-1a/Pou2f3 specifies taste receptor cell lineage. Nat. Neurosci. 2011; 14:685-7.
Zhang Z, Huettner PC, Nguyen L, et al. Aberrant promoter methylation and silencing of the POU2F3 gene in cervical cancer. Oncogene 2006; 25:5436-45.
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