癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
NDST2 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E |
|---|---|
| Primary Accession | P52849 |
| Other Accession | NP_003626, 4505353 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 100875 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | NDST2 antibody can be used for detection of PDCL by Western blot at 1 - 2 µg/ml. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 8509 |
|---|---|
| Other Names | Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2, 2.8.2.8, Glucosaminyl N-deacetylase/N-sulfotransferase 2, NDST-2, N-heparan sulfate sulfotransferase 2, N-HSST 2, Heparan sulfate N-deacetylase 2, 3.-.-.-, Heparan sulfate N-sulfotransferase 2, 2.8.2.-, NDST2, HSST2 |
| Target/Specificity | NDST2; NDST2 antibody is human, mouse and rat reactive. At least two isoforms of NDST2 are known to exist; this antibody will only detect the larger isoform. NDST2 antibody is predicted not to cross-react with other members of the NDST protein family. |
| Reconstitution & Storage | NDST2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | NDST2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | NDST2 |
|---|---|
| Synonyms | HSST2 |
| Function | Essential bifunctional enzyme that catalyzes both the N- deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate (By similarity). Exhibits N- deacetylase activity (PubMed:16343444). Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis (By similarity). Plays a role in determining the extent and pattern of sulfation of heparan sulfate (By similarity). Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). Essential for the biosynthesis of sulfated heparin in mast cells, thereby enabling proper secretory granule formation and storage of mast-cell proteases (By similarity). |
| Cellular Location | Golgi apparatus membrane {ECO:0000250|UniProtKB:Q02353}; Single-pass type II membrane protein |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2 (NDST2) protein, also known as N-heparan sulfate sulfotransferase 2, and the highly related protein NDST1 have key roles in the sulfation of heparan sulfate proteoglycans, molecules that are present of cell surfaces and the extracellular matrix (1,2). NDST1 and NDST2 are dispensable for mesodermal differentiation into osteoblasts but necessary for induction of adipocytes and neural cells (3).
REFERENCES
Humphries DE, Lanciotti J, and Karlinsky JB. cDNA cloning, genomic organization and chromosomal localization of human heparan glucosaminyl N-deacetylase/N-sulphotransferase-2. Biochem. J. 1998; 332:303-7.
Esko JD and Selleck SB. Order out of chaos. Assembly of ligand binding sites in heparan sulfate. Annu. Rev. Biochem. 2002; 71:435-71.
Forsberg M, Holmborn K, Kundu S, et al. Undersulfation of heparan sulfate restricts differentiation potential of mouse embryonic stem cells. J. Biol. Chem. 2012; 287:10853-62.
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