癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
PIK3R4 Antibody
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- 背景知识
Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | Q99570 |
| Other Accession | NP_055417, 23943912 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 153103 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | PIK3R4 antibody can be used for detection of PIK3R4 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 30849 |
|---|---|
| Other Names | Phosphoinositide 3-kinase regulatory subunit 4, PI3-kinase regulatory subunit 4, 2.7.11.1, PI3-kinase p150 subunit, Phosphoinositide 3-kinase adaptor protein, PIK3R4 |
| Target/Specificity | PIK3R4; PIK3R4 antibody is human, mouse and rat reactive. |
| Reconstitution & Storage | PIK3R4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | PIK3R4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | PIK3R4 |
|---|---|
| Synonyms | VPS15 {ECO:0000303|PubMed:23878393} |
| Function | Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123). |
| Cellular Location | Late endosome. Cytoplasmic vesicle, autophagosome. Membrane; Lipid-anchor. Note=As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes. Localizes also to discrete punctae along the ciliary axoneme (By similarity) {ECO:0000250|UniProtKB:Q8VD65, ECO:0000305} |
| Tissue Location | Ubiquitously expressed. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
PIK3R4 (phosphoinositide-3-kinase regulatory subunit 4, also known as VPS15) is a 150kD adaptor protein that enhances the lipid kinase activity of PIK3 complexes and may be involved in regulating membrane trafficking late in the endocytic pathway and autophagy (1,2). PIK3R4 is ubiquitously expressed and contains 7 WD repeats, 3 HEAT repeats and 1 protein kinase domain (1). PIK3R4 is crucial for VPS34 function and is necessary for autophagosome formation in multicellular animals and reveal an important role of VPS15 in cellular protection against ubiquitin-positive protein aggregates (3-5).
REFERENCES
Herman PK, Stack JH, and Emr SD. A genetic and structural analysis of the yeast Vps15 protein kinase: evidence for a direct role of Vps15p in vacuolar protein delivery. EMBO J. 1991; 10:4049-60.
Stasyk OV, van der Klei IJ, Bellu AR, et al. A Pichia pastoris VPS15 homologue is required in selective peroxisome autophagy. Curr. Genet. 1999; 36:262-9.
Kihara A, Noda T, Ishihara N, et al. Two distinct Vps34 phosphatidylinositol 3-kinase complexes function in autophagy and carboxypeptidase Y sorting in Saccharomyces cerevisiae. J. Cell Biol. 2001; 152:519-30.
Stack JH, DeWald DB, Takegawa K, et al. Vesicle-mediated protein transport: regulatory interactions between the Vps15 protein kinase and the Vps34 PtdIns 3-kinase essential for protein sorting to the vacuole in yeast. J. Cell Biol. 1995; 129:321-34.
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