癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
RHOT1 Antibody
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Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | Q8IXI2 |
| Other Accession | NP_001028740, 75750480 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 70784 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | RHOT1 antibody can be used for detection of RHOT1 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 55288 |
|---|---|
| Other Names | Mitochondrial Rho GTPase 1, MIRO-1, hMiro-1, 3.6.5.-, Rac-GTP-binding protein-like protein, Ras homolog gene family member T1, RHOT1, ARHT1 |
| Target/Specificity | RHOT1; RHOT1 antibody is human, mouse and rat reactive. At least three isoforms of RHOT1 are known to exist; this antibody will detect all three isoforms. |
| Reconstitution & Storage | RHOT1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | RHOT1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RHOT1 |
|---|---|
| Synonyms | ARHT1 |
| Function | Atypical mitochondrial nucleoside-triphosphatase (NTPase) involved in mitochondrial trafficking (PubMed:12482879, PubMed:16630562, PubMed:22396657, PubMed:30513825). Probably involved in control of anterograde transport of mitochondria and their subcellular distribution (PubMed:12482879, PubMed:16630562, PubMed:22396657). Promotes mitochondrial fission during high calcium conditions (PubMed:27716788). Can hydrolyze GTP, ATP and UTP (PubMed:30513825). |
| Cellular Location | Mitochondrion outer membrane; Single-pass type IV membrane protein. Note=Colocalizes with MGARP and RHOT2 at the mitochondria |
| Tissue Location | Ubiquitously expressed. Expressed at high level in heart and skeletal muscle. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The Ras homolog family member T1 (RHOT) is an atypical Rho Ca2+-binding GTPase that localizes to the mitochondria (1). RHOT1, the related protein RHOT2, the adaptor protein Milton, and the PTEN induced putative kinase 1 (PINK1), form a complex that is involved in axonal transport of mitochondria (2,3). Both PINK1 and Parkin target RHOT1 for phosphorylation and degradation, causing the arrest of mitochondrial motility (4).
REFERENCES
Fransson A, Ruusala A, and Aspenstrom P. Atypical Rho GTPases have roles in mitochondrial homeostasis and apoptosis. J. Biol. Chem. 2003; 278:6495-502.
Macaskill AF, Brickley K, Stephenson FA, et al. GTPase dependent recruitment of Grif-1 by Miro1 regulates mitochondrial trafficking in hippocampal neurons. Mol. Cell. Neurosci. 2009;40:301–12.
Weihofen A, Thomas KJ, Ostaszewski B, et al. Pink1 forms a multi-protein complex with Miro and Milton, linking Pink1 function to mitochondrial trafficking. Biochemistry 2009; 48:2045-54.
Wang X, Winter D, Ashrafi G, et al. PINK1 and Parkin target Miro for phosphorylation and degradation to arrest mitochondrial motility. Cell 2011; 147:893-906.
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