癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
NOSTRIN Antibody
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- 实验流程
- 背景知识
Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | Q8IVI9 |
| Other Accession | NP_443178, 284172479 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 57660 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | NOSTRIN antibody can be used for detection of NOSTRIN by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 115677 |
|---|---|
| Other Names | Nostrin, BM247 homolog, Nitric oxide synthase traffic inducer, Nitric oxide synthase trafficker, eNOS-trafficking inducer, NOSTRIN |
| Target/Specificity | NOSTRIN; NOSTRIN antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect all four isoforms. |
| Reconstitution & Storage | NOSTRIN antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | NOSTRIN Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | NOSTRIN (HGNC:20203) |
|---|---|
| Function | Multivalent adapter protein which may decrease NOS3 activity by inducing its translocation away from the plasma membrane. |
| Cellular Location | [Isoform 1]: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle. Cytoplasm, cytoskeleton Note=Enriched in selected actin structures (PubMed:16234328, PubMed:16376344) |
| Tissue Location | Expressed at highest levels in heart, kidney, placenta and lung, and at lowest levels in brain, thymus and spleen Present in vascular endothelial cells and placenta. Over-expressed in placenta from women with pre-eclampsia (at protein level) |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
NOSTRIN. also known as endothelial nitric oxide synthase traffic inducer, is a member of the Pombe Cdc15 homology (PCH) family of proteins which may function as the modulator of the subcellular localisation and activity of endothelial nitric oxide synthase (eNOS) (1,2). It decreases NOS3 activity by inducing its translocation away from the plasma membrane (3). NOSTRIN typically exists as a trimer and is expressed in the vascular endothelial cells of highly vascularized tissues such as placenta, lung, kidney and heart. NOSTRIN is necessary for proper vascular development in zebrafish and postnatal retinal angiogenesis in mice (4,5).
REFERENCES
Zimmermann K, Opitz N, Dedio J, et al. NOSTRIN: a protein modulating nitric oxide release and subcellular distribution of endothelial nitric oxide synthase. Proc. Natl. Acad. Sci. USA 2002; 99:17167-72.
Icking A, Matt S, Opitz N, et al. NOSTRIN functions as a homotrimeric adaptor protein facilitating internalization of eNOS. J. Cell Sci. 2005; 118:5059-69.
Schilling K, Opitz N, Wiesenthal A, et al. Translocation of endothelial nitric-oxide synthase involves a ternary complex with caveolin-1 and NOSTRIN. Mol. Biol. Cell 2006; 17:3870-80.
Icking A, Schilling K, Wiesenthal A, et al. FCH/Cdc15 domain determines distinct subcellular localization of NOSTRIN. FEBS Lett. 2006; 580:223-8.
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