癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
DIRAS3 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | O95661 |
| Other Accession | NP_004666, 4757772 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 25861 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | DIRAS3 antibody can be used for detection of DIRAS3 by Western blot at 1 - 2 µg/ml. |
| Gene ID | 9077 |
|---|---|
| Other Names | GTP-binding protein Di-Ras3, Distinct subgroup of the Ras family member 3, Rho-related GTP-binding protein RhoI, DIRAS3, ARHI, NOEY2, RHOI |
| Target/Specificity | DIRAS3; DIRAS3 antibody is human specific. |
| Reconstitution & Storage | DIRAS3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | DIRAS3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DIRAS3 |
|---|---|
| Synonyms | ARHI, NOEY2, RHOI |
| Cellular Location | Cell membrane; Lipid-anchor; Cytoplasmic side |
| Tissue Location | Expressed in normal ovarian and breast epithelial cells but not in ovarian and breast cancers |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
DIRAS3 is a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with mono-allelic expression of the paternal allele, which is associated with growth suppression and down-regulation of cyclin D1 promoter activity and induction of p21 (WAF/CIP1). Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers (1). DIRAS3 has been shown to induce autophagy in human ovarian cancer cells by blocking PI3K signaling, inhibiting the mammalian target of rapamycin (TOR), upregulating ATG4, and colocalizing with LC3 in autophagosomes (2). DIRAS also interacts with C-RAF and downregulates mitogen-activated protein kinase kinases (MEK) to restrict cell migration (3).
REFERENCES
Yu Y, Xu F, Peng H, et al. NOEY2 (ARHI), an imprinted putative tumor suppressor gene in ovarian and breast carcinomas. Proc. Natl. Acad. Sci. USA 1999; 96:214-9.
Lu Z, Luo RZ, Lu Y, et al. The tumor suppressor gene ARHI regulates autophagy and tumor dormancy in human ovarian cancer cells. J. Clin. Invest. 2008; 118:3917-29.
Klingauf M, Beck M, Berge U, et al. The tumour suppressor DiRas3 interacts with C-RAF and downregulates MEK activity to restrict cell migration. Biol. Cell 2013; 105:91-107.
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