癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
PMEPA1 Antibody
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- 实验流程
- 背景知识
Application 
| WB, IHC, E |
|---|---|
| Primary Accession | Q969W9 |
| Other Accession | NP_064567, 21361841 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 31609 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | PMEPA1 antibody can be used for detection of PMEPA1 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. |
| Gene ID | 56937 |
|---|---|
| Other Names | Protein TMEPAI, Prostate transmembrane protein androgen induced 1, Solid tumor-associated 1 protein, Transmembrane prostate androgen-induced protein, PMEPA1, STAG1, TMEPAI |
| Target/Specificity | PMEPA1; PMEPA1 antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect all of the isoforms. |
| Reconstitution & Storage | PMEPA1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | PMEPA1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | PMEPA1 |
|---|---|
| Synonyms | STAG1, TMEPAI |
| Function | Functions as a negative regulator of TGF-beta signaling and thereby probably plays a role in cell proliferation, differentiation, apoptosis, motility, extracellular matrix production and immunosuppression. In the canonical TGF-beta pathway, ZFYVE9/SARA recruits the intracellular signal transducer and transcriptional modulators SMAD2 and SMAD3 to the TGF-beta receptor. Phosphorylated by the receptor, SMAD2 and SMAD3 then form a heteromeric complex with SMAD4 that translocates to the nucleus to regulate transcription. Through interaction with SMAD2 and SMAD3, LDLRAD4 may compete with ZFYVE9 and SMAD4 and prevent propagation of the intracellular signal (PubMed:20129061, PubMed:24627487). Also involved in down-regulation of the androgen receptor (AR), enhancing ubiquitination and proteasome- mediated degradation of AR, probably by recruiting NEDD4 (PubMed:18703514). |
| Cellular Location | Early endosome membrane; Single-pass membrane protein. Golgi apparatus membrane; Single-pass membrane protein |
| Tissue Location | Highest expression in prostate. Also expressed in ovary |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The prostate transmembrane protein, androgen induced 1 (PMEPA1) protein is a transmembrane protein that contains a Smad interacting motif (SIM) (1,2). Expression of this gene is induced by androgens and transforming growth factor beta, and the encoded protein suppresses the androgen receptor and transforming growth factor beta signaling pathways though interactions with Smad proteins (3). Overexpression of this gene may play a role in multiple types of cancer (2,4).
REFERENCES
Xu LL, Shanmugam N, Segawa T, et al. A novel androgen-regulated gene, PMEPA1, located on chromosome 20q!3 exhibits high level expression in prostate. Genomics 2000; 66:257-63.
Rae FK, Hooper JD, Nicol DL, et al. Characterization of a novel gene, STAG1/PMEPA1, upregulated in renal cell carcinoma and other solid tumors. Mol. Carcinog. 2001; 32:44-53.
Watanabe Y, Itoh S, Goto T, et al. TMEPAI, a transmembrane TGF-beta-inducible protein, sequesters Smad proteins from active participation in TGF-beta signaling. Mol. Cell 2010; 37:123-34.
Vo Nguyen TT, Watanabe Y, Shiba A, et al. TMEPAI/PMEPA1 enhances tumorigenic activities in lung cancer cells. Cancer Sci. 2014; 105:334-41.
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