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Anti-Swine IgG (H&L) Secondary Antibody

Goat Polyclonal, Unconjugated

     
  • 1 - Anti-Swine IgG (H&L)  Secondary Antibody ASR1126
    Western Blot of Goat anti-Swine IgG antibody. Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: Swine Antibody at 1:1,000 o/n at 4°C . Secondary antibody: DyLight™ 649 goat secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Swine IgG. Other band(s): none.
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Product Information
Description Anti-SWINE IgG (H&L) (GOAT) Antibody
Host Goat
Conjugate Unconjugated
Target Species Swine
Clonality Polyclonal
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Physical State Liquid (sterile filtered)
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Swine IgG (H&L) was produced by repeated immunization with swine whole IgG molecule in goat.
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Application Note Anti-Swine IgG (H&l) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Swine IgG and Swine Serum.
Storage Condition Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Anti-Swine IgG secondary antibody generated in goat detects specifically swine IgG. This secondary antibody anti-Swine is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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