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Anti-Human IgG (H&L) Secondary Antibody

Mouse Polyclonal, Unconjugated

     
  • 1 - Anti-Human IgG (H&L)  Secondary Antibody ASR1599
    Western Blot of Human IgG Antibody. M: 3 µL Opal Prestained Ladder (MB-210-0500). Lane 1: Human IgG. Load: 50 ng per lane. Primary antibody: Human IgG Antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase conjugated mouse secondary antibody at 1:40,000 for 30 minutes at RT. Block: MB-070 for 30 minutes at RT. Predicted/Observed size: 55 kDa, 25 kDa for Human IgG.
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Product Information
Description Anti-HUMAN IgG (H&L) (MOUSE) Antibody
Host Mouse
Conjugate Unconjugated
Target Species Human
Reactivity Human
Clonality Polyclonal
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Physical State Liquid (sterile filtered)
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Human IgG whole molecule
Stabilizer None
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity This product was prepared from monospecific polyclonal ascites by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgG and Human Serum.
Storage Condition Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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