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Mouse IgG2b isotype control Phycoerythrin

Monoclonal M2B IgG2b , R-Phycoerythrin (RPE)

     
  • 6 - Mouse IgG2b isotype control Phycoerythrin ASR3078
    Dot Blot of Mouse IgG2b Isotype Control Phycoerythrin Conjugated. Antigen: none. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Mouse IgG2b Isotype Control Phycoerythrin Conjugated blotted directly to membrane at above dilutions. Block: MB-070 for 60 min at RT.
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Product Information
Description MOUSE IgG2b isotype control Phycoerythrin conjugated
Conjugate R-Phycoerythrin (RPE)
Clonality Monoclonal M2B IgG2b
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
DB
Physical State Lyophilized
Host Isotype IgG2b
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Species of Origin Mouse
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Application Note Mouse IgG2b isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Purity This product was prepared from ascitic fluid by affinity chromatography.   Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Phycoerythrin and Anti-Mouse Serum.  Specificity was confirmed by ELISA at less than 1% cross reactivity against other anti-Mouse heavy or light chain isotypes antibodies.
Storage Condition Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.

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