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FZR1 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a full-length recombinant FZR1.

Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (80.08 KDa) .
FZR1 monoclonal antibody (M02), clone 4C4. Western Blot analysis of FZR1 expression in Hela S3 NE.
Immunoperoxidase of monoclonal antibody to FZR1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
Detection limit for recombinant GST tagged FZR1 is 1 ng/ml as a capture antibody.
Proximity Ligation Analysis of protein-protein interactions between CCNB1 and FZR1. HeLa cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-FZR1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Primary Accession	Q9UM11
Other Accession	BC013413
Reactivity	Human
Host	mouse
Clonality	monoclonal
Isotype	IgG2a Kappa
Clone Names	4C4
Calculated MW	55179 Da

Additional Information
Gene ID	51343
Other Names	Fizzy-related protein homolog, Fzr, CDC20-like protein 1, Cdh1/Hct1 homolog, hCDH1, FZR1, CDH1, FYR, FZR, KIAA1242
Target/Specificity	FZR1 (AAH13413.1, 1 a.a. ~ 493 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution	WB~~1:500~1000 IHC~~1:100~500 E~~N/A
Format	Clear, colorless solution in phosphate buffered saline, pH 7.2 .
Storage	Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Precautions	FZR1 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

The anaphase-promoting complex/cyclosome activator Cdh1 modulates Rho GTPase by targeting p190 RhoGAP for degradation. Naoe H, et al. Mol Cell Biol, 2010 Aug. PMID 20530197.Centrosome-related genes, genetic variation, and risk of breast cancer. Olson JE, et al. Breast Cancer Res Treat, 2010 May 28. PMID 20508983.Loss of the mammalian APC/C activator FZR1 shortens G1 and lengthens S phase but has little effect on exit from mitosis. Sigl R, et al. J Cell Sci, 2009 Nov 15. PMID 19861496.The Cdc14B-Cdh1-Plk1 axis controls the G2 DNA-damage-response checkpoint. Bassermann F, et al. Cell, 2008 Jul 25. PMID 18662541.Dissection of the APCCdh1-Skp2 cascade in breast cancer. Fujita T, et al. Clin Cancer Res, 2008 Apr 1. PMID 18381934.

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¥ 3,700.00

Cat# AT2132a

Size:

100 µg

Price:

¥ 3,700.00

Quantity:

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Availability: 6-8 weeks

询价

FZR1 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a full-length recombinant FZR1.

REFERENCES

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