癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
GCLC Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant GCLC.
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E |
|---|---|
| Primary Accession | P48506 |
| Other Accession | NM_001498 |
| Reactivity | Human, Mouse, Rat |
| Host | mouse |
| Clonality | monoclonal |
| Isotype | IgG1 Kappa |
| Clone Names | 3H1 |
| Calculated MW | 72766 Da |
| Gene ID | 2729 |
|---|---|
| Other Names | Glutamate--cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase, GCLC, GLCL, GLCLC |
| Target/Specificity | GCLC (NP_001489, 528 a.a. ~ 637 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 IF~~1:50~200 E~~N/A |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | GCLC Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.
REFERENCES
1.Prominent steatosis with hypermetabolism of the cell line permissive for years of infection with hepatitis C virus.Sugiyama K, Ebinuma H, Nakamoto N, Sakasegawa N, Murakami Y, Chu PS, Usui S, Ishibashi Y, Wakayama Y, Taniki N, Murata H, Saito Y, Fukasawa M, Saito K, Yamagishi Y, Wakita T, Takaku H, Hibi T, Saito H, Kanai TPLoS One. 2014 Apr 9;9(4):e94460. doi: 10.1371/journal.pone.0094460. eCollection 2014.2.The LEGSKO Mouse: A Mouse Model of Age-Related Nuclear Cataract Based on Genetic Suppression of Lens Glutathione Synthesis.Fan X, Liu X, Hao S, Wang B, Robinson ML, Monnier VM.PLoS One. 2012;7(11):e50832. doi: 10.1371/journal.pone.0050832. Epub 2012 Nov 30.3.Differential activation of the inflammasome in THP-1 cells exposed to chrysotile asbestos and Libby"six-mix"amphiboles and subsequent activation of BEAS-2B cells.Li M, Gunter ME, Fukagawa NK.Cytokine. 2012 Sep 24. pii: S1043-4666(12)00666-7. doi: 10.1016/j.cyto.2012.08.025.4.Protection against 2-chloroethyl ethyl sulfide (CEES) - Induced cytotoxicity in human keratinocytes by an inducer of the glutathione detoxification pathway.Abel EL, Bubel JD, Simper MS, Powell L, McClellan SA, Andreeff M, Macleod MC, Digiovanni J.Toxicol Appl Pharmacol. 2011 Jun 23. [Epub ahead of print]5.Diversity in Antioxidant Response Enzymes in Progressive Stages of Human Nonalcoholic Fatty Liver Disease.Hardwick RN, Fisher CD, Canet MJ, Lake AD, Cherrington NJ.Drug Metab Dispos. 2010 Dec;38(12):2293-301. Epub 2010 Aug 30.
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