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>   首页   >   产品   >   一抗   >   代谢   >   PYCRL Antibody (monoclonal) (M01)   

PYCRL Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant PYCRL.

     
  • 1 - PYCRL Antibody (monoclonal) (M01) AT3505a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
  • 1 - PYCRL Antibody (monoclonal) (M01) AT3505a
    PYCRL monoclonal antibody (M01), clone 4F11 Western Blot analysis of PYCRL expression in HeLa ( (Cat # AT3505a )
  • 1 - PYCRL Antibody (monoclonal) (M01) AT3505a
    Western Blot analysis of PYCRL expression in transfected 293T cell line by PYCRL monoclonal antibody (M01), clone 4F11.

    Lane 1: PYCRL transfected lysate (Predicted MW: 28.6 KDa).
    Lane 2: Non-transfected lysate.
  • 3 - PYCRL Antibody (monoclonal) (M01) AT3505a
    Immunofluorescence of monoclonal antibody to PYCRL on HeLa cell. [antibody concentration 10 ug/ml]
  • 5 - PYCRL Antibody (monoclonal) (M01) AT3505a
    Immunoprecipitation of PYCRL transfected lysate using anti-PYCRL monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PYCRL monoclonal antibody.
    U0007), and immunoblotted with PYCRL monoclonal antibody." href="/assets/uploads/products/200811/AT3505a_IP_1.jpg">
  • 10 - PYCRL Antibody (monoclonal) (M01) AT3505a
    Detection limit for recombinant GST tagged PYCRL is approximately 0.3ng/ml as a capture antibody.
  • 产品详情
  • 实验流程
Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IP, E
Primary Accession Q53H96
Other Accession NM_023078
Reactivity Human
Host mouse
Clonality monoclonal
Isotype IgG2a Kappa
Clone Names 4F11
Calculated MW 28663 Da
Additional Information
Gene ID 65263
Other Names Pyrroline-5-carboxylate reductase 3, P5C reductase 3, P5CR 3, Pyrroline-5-carboxylate reductase-like protein, PYCRL
Target/Specificity PYCRL (NP_075566, 32 a.a. ~ 131 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
IF~~1:50~200
IP~~N/A
E~~N/A
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPYCRL Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

REFERENCES

1.Functional specialization in proline biosynthesis of melanoma.De Ingeniis J, Ratnikov B, Richardson AD, Scott DA, Aza-Blanc P, De SK, Kazanov M, Pellecchia M, Ronai Z, Osterman AL, Smith JW.PLoS One. 2012;7(9):e45190. Epub 2012 Sep 14.

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