癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
SPAST Antibody (monoclonal) (M02)
Mouse monoclonal antibody raised against a partial recombinant SPAST.
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IHC, IF |
|---|---|
| Primary Accession | Q9UBP0 |
| Other Accession | NM_014946 |
| Reactivity | Human |
| Host | mouse |
| Clonality | monoclonal |
| Isotype | IgG1 Kappa |
| Clone Names | 2F5 |
| Calculated MW | 67197 Da |
| Gene ID | 6683 |
|---|---|
| Other Names | Spastin {ECO:0000255|HAMAP-Rule:MF_03021}, 3643 {ECO:0000255|HAMAP-Rule:MF_03021}, Spastic paraplegia 4 protein, SPAST {ECO:0000255|HAMAP-Rule:MF_03021} |
| Target/Specificity | SPAST (NP_055761, 200 a.a. ~ 304 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 IHC~~1:100~500 IF~~1:50~200 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | SPAST Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This gene encodes a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. The encoded ATPase may be involved in the assembly or function of nuclear protein complexes. Two transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their full length sequences have not been determined. Mutations associated with this gene cause the most frequent form of autosomal dominant spastic paraplegia 4.
REFERENCES
Unique spectrum of SPAST variants in Estonian HSP patients: presence of benign missense changes but lack of exonic rearrangements. Braschinsky M, et al. BMC Neurol, 2010 Mar 9. PMID 20214791.Hereditary spastic paraplegia proteins REEP1, spastin, and atlastin-1 coordinate microtubule interactions with the tubular ER network. Park SH, et al. J Clin Invest, 2010 Apr 1. PMID 20200447.Functional conservation of human Spastin in a Drosophila model of autosomal dominant-hereditary spastic paraplegia. Du F, et al. Hum Mol Genet, 2010 May 15. PMID 20154342.A novel splicing mutation (c.870+3A>G) in SPG4 in a Korean family with hereditary spastic paraplegia. Lim JS, et al. J Neurol Sci, 2010 Mar 15. PMID 19939411.Mutation analysis of the SPG4 gene in Italian patients with pure and complicated forms of spastic paraplegia. Magariello A, et al. J Neurol Sci, 2010 Jan 15. PMID 19875132.
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