癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
WWP1 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant WWP1.
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IHC, IF, IP, E |
|---|---|
| Primary Accession | Q9H0M0 |
| Other Accession | NM_007013 |
| Reactivity | Human |
| Host | mouse |
| Clonality | monoclonal |
| Isotype | IgG2a Kappa |
| Clone Names | 1A7 |
| Calculated MW | 105202 Da |
| Gene ID | 11059 |
|---|---|
| Other Names | NEDD4-like E3 ubiquitin-protein ligase WWP1, 632-, Atrophin-1-interacting protein 5, AIP5, TGIF-interacting ubiquitin ligase 1, Tiul1, WW domain-containing protein 1, WWP1 |
| Target/Specificity | WWP1 (NP_008944, 152 a.a. ~ 260 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 IHC~~1:100~500 IF~~1:50~200 IP~~N/A E~~N/A |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | WWP1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants; however, the full length nature of these transcripts has not been defined.
REFERENCES
1.Knockdown of WWP1 inhibits growth and induces apoptosis in hepatoma carcinoma cells through the activation of caspase3 and p53.Cheng Q, Cao X, Yuan F, Li G, Tong TBiochem Biophys Res Commun. 2014 May 1. pii: S0006-291X(14)00780-3. doi: 10.1016/j.bbrc.2014.04.117.2.WWP1 delays cellular senescence by promoting p27Kip1 degradation in human diploid fibroblasts.Cao X, Xue L, Han L, Ma L, Chen T, Tong T.J Biol Chem. 2011 Jul 27. [Epub ahead of print]3.The E3 ubiquitin ligase WWP1 regulates ?GNp63-dependent transcription through Lys63 linkages.Peschiaroli A, Scialpi F, Bernassola F, Sherbini el SE, Melino G.Biochem Biophys Res Commun. 2010 Nov 12;402(2):425-30. Epub 2010 Oct 15.4.Endogenous spartin (SPG20) is recruited to endosomes and lipid droplets and interacts with the ubiquitin E3 ligases AIP4 and AIP5.Edwards TL, Clowes VE, Tsang HT, Connell JW, Sanderson CM, Luzio JP, Reid E.Biochem J. 2009 Sep 14;423(1):31-9.
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