Musk Antibody
Purified Mouse Monoclonal Antibody (Mab)
- 产品详情
- 实验流程
- 背景知识
Application ![]()
| IHC-P, WB |
---|---|
Primary Accession | Q61006 |
Reactivity | Rat |
Predicted | Mouse |
Host | Mouse |
Clonality | monoclonal |
Calculated MW | 96693 Da |
Isotype | IgG1,k |
Antigen Source | HUMAN |
Gene ID | 18198 |
---|---|
Other Names | Muscle, skeletal receptor tyrosine-protein kinase, Muscle-specific tyrosine-protein kinase receptor, MuSK, Muscle-specific kinase receptor, Musk, Nsk2 |
Dilution | IHC-P~~1:100~500 WB~~1:1000 |
Target/Specificity | This mouse Musk antibody is generated from a mouse immunized with recombinant protein from mouse Musk. |
Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Musk Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Musk |
---|---|
Synonyms | Nsk2 |
Function | Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation. |
Cellular Location | Postsynaptic cell membrane; Single-pass type I membrane protein. Note=Localizes to the postsynaptic cell membrane of the neuromuscular junction |
Tissue Location | Expressed preferentially in skeletal muscle. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation.
REFERENCES
Caruso A.,et al.Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases.
Ganju P.,et al.Oncogene 11:281-290(1995).
DeChiara T.M.,et al.Cell 85:501-512(1996).
Glass D.J.,et al.Cell 85:513-523(1996).
Lin W.,et al.Nature 410:1057-1064(2001).

终于等到您。ABCEPTA(百远生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。
如有疑问,联系:0512-88856768 tech-china@abcepta.com.
刘
2017-07-10 15:32:19
1
2
3
4
5
|
Species tested
小鼠
Application tested
WB稀释比例1:700 一抗4°过夜;二抗1:500室温1h
Organization tested
小鼠心肌细胞
Barcode encoding
Brief protocol
|