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>   首页   >   产品   >   一抗   >   心血管   >   EPOR Antibody (C-term)   

EPOR Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - EPOR Antibody (C-term) AW5363
    Overlay histogram showing K562 cells stained with AW5363(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AW5363, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - EPOR Antibody (C-term) AW5363
    All lanes : Anti-EPOR Antibody (C-term) at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB
Primary Accession P19235
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 55065 Da
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 2057
Antigen Region 470-504 aa
Other Names Erythropoietin receptor, EPO-R, EPOR
Dilution FC~~1:25
WB~~1:2000
Target/Specificity This EPOR antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 470-504 amino acids from the C-terminal region of human EPOR.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEPOR Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EPOR {ECO:0000303|PubMed:2163695, ECO:0000312|HGNC:HGNC:3416}
Function Receptor for erythropoietin, which mediates erythropoietin- induced erythroblast proliferation and differentiation (PubMed:10388848, PubMed:2163695, PubMed:2163696, PubMed:8662939, PubMed:9774108). Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade (By similarity). In some cell types, can also activate STAT1 and STAT3 (PubMed:11756159). May also activate the LYN tyrosine kinase (By similarity).
Cellular Location Cell membrane {ECO:0000250|UniProtKB:P14753}; Single-pass type I membrane protein
Tissue Location Erythroid cells and erythroid progenitor cells. [Isoform EPOR-S]: Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Receptor for erythropoietin. Mediates erythropoietin- induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.

REFERENCES

Winkelmann J.C.,et al.Blood 76:24-30(1990).
Jones S.S.,et al.Blood 76:31-35(1990).
Noguchi C.T.,et al.Blood 78:2548-2556(1991).
Ehrenman K.,et al.Exp. Hematol. 19:973-977(1991).
Nakamura Y.,et al.Science 257:1138-1141(1992).

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