CCL15 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

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AW5472 staining CCL15 in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Overlay histogram showing MCF-7 cells stained with AW5472 (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody ( , 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-CCL15 Antibody at 1:1000 dilution Lane 1: human heart lysates Lane 2: human liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 12 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IHC-P
Primary Accession	Q16663
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	12236 Da
Isotype	Rabbit IgG
Antigen Source	HUMAN

Additional Information
Gene ID	6359
Other Names	C-C motif chemokine 15, Chemokine CC-2, HCC-2, Leukotactin-1, LKN-1, MIP-1 delta, Macrophage inflammatory protein 5, MIP-5, Mrp-2b, NCC-3, Small-inducible cytokine A15, CCL15(22-92), CCL15(25-92), CCL15(29-92), CCL15, MIP5, NCC3, SCYA15
Dilution	WB~~1:1000 FC~~1:25 IHC-P~~1:100~500
Target/Specificity	This CCL15 antibody is generated from a rabbit immunized with a recombinant protein.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CCL15 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CCL15
Synonyms	MIP5, NCC3, SCYA15
Function	Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the CCL15.
Cellular Location	Secreted.
Tissue Location	Most abundant in heart, skeletal muscle and adrenal gland. Lower levels in placenta, liver, pancreas and bone marrow CCL15(22-92), CCL15(25-92) and CCL15(29-92) are found in high levels in synovial fluids from rheumatoid patients.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15.

REFERENCES

Youn B.-S.,et al.J. Immunol. 159:5201-5205(1997).
Wang W.,et al.J. Clin. Immunol. 18:214-222(1998).
Pardigol A.,et al.Proc. Natl. Acad. Sci. U.S.A. 95:6308-6313(1998).
Nomiyama H.,et al.J. Interferon Cytokine Res. 19:227-234(1999).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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¥ 2,600.00

Cat# AW5472

Size:

100 µl

Price:

¥ 2,600.00

Quantity:

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Availability: In Stock

询价

CCL15 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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