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RPS15 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - RPS15 Antibody (N-term) AW5522
    All lanes : Anti-RPS15 Antibody (N-term) at 1:1000 dilution Lane 1: U-87 MG whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - RPS15 Antibody (N-term) AW5522
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with RPS15 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - RPS15 Antibody (N-term) AW5522
    Flow cytometric analysis of HepG2 cells using RPS15 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IHC-P, WB
Primary Accession P62841
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 17040 Da
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 6209
Antigen Region 4-38 aa
Other Names 40S ribosomal protein S15, RIG protein, RPS15, RIG
Dilution FC~~1:10~50
IHC-P~~1:100~500
WB~~1:1000
Target/Specificity This RPS15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-38 amino acids from the N-terminal region of human RPS15.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPS15 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name RPS15
Synonyms RIG
Function Component of the small ribosomal subunit (PubMed:23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399).
Cellular Location Cytoplasm.

BACKGROUND

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS15 is a component of the 40S subunit. The protein belongs to the S19P family of ribosomal proteins. It is located in the cytoplasm.

REFERENCES

Yu,Y., et.al., Protein Sci. 14 (6), 1438-1446 (2005)

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