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RPS15 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - RPS15 Antibody (N-term) AP6914a
    Western blot analysis of RPS15 Antibody (N-term) (Cat. #AP6914a) in HepG2 cell line lysates (35ug/lane). RPS15 (arrow) was detected using the purified Pab.
  • 14 - RPS15 Antibody (N-term) AP6914a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with RPS15 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - RPS15 Antibody (N-term) AP6914a
    Flow cytometric analysis of HepG2 cells using RPS15 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession P62841
Other Accession P62845, P62844, P62843, P62846, Q56K10
Reactivity Human, Rat, Mouse
Predicted Bovine, Chicken, Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 17040 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 6209
Other Names 40S ribosomal protein S15, RIG protein, RPS15, RIG
Target/Specificity This RPS15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RPS15.
Dilution WB~~1:1000
IHC-P~~1:100~500
FC~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPS15 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPS15
Synonyms RIG
Function Component of the small ribosomal subunit (PubMed:23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399).
Cellular Location Cytoplasm.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS15 is a component of the 40S subunit. The protein belongs to the S19P family of ribosomal proteins. It is located in the cytoplasm.

REFERENCES

Yu,Y., et.al., Protein Sci. 14 (6), 1438-1446 (2005)

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