PECI Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

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All lanes : Anti-PECI Antibody (C-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PECI Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
PECI Antibody (C-term) (Cat. #AW5533) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, IHC-P, WB
Primary Accession	O75521
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	43585 Da
Isotype	Rabbit IgG
Antigen Source	HUMAN

Additional Information
Gene ID	10455
Antigen Region	331-358 aa
Other Names	Enoyl-CoA delta isomerase 2, mitochondrial, DRS-1, Delta(3), delta(2)-enoyl-CoA isomerase, D3, D2-enoyl-CoA isomerase, Diazepam-binding inhibitor-related protein 1, DBI-related protein 1, Dodecenoyl-CoA isomerase, Hepatocellular carcinoma-associated antigen 88, Peroxisomal 3, 2-trans-enoyl-CoA isomerase, pECI, Renal carcinoma antigen NY-REN-1, ECI2, DRS1, HCA88, PECI
Dilution	FC~~1:10~50 IHC-P~~1:100~500 WB~~1:1000
Target/Specificity	This PECI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-358 amino acids from the C-terminal region of human PECI.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	PECI Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ECI2
Synonyms	DRS1, HCA88, PECI {ECO:0000303\|PubMed:10
Function	Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates.
Cellular Location	[Isoform 1]: Mitochondrion {ECO:0000250\|UniProtKB:Q5XIC0}
Tissue Location	Abundant in heart, skeletal muscle and liver. Expressed in CD34(+) T-cells and CD34(+) bone marrow cells

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.