PECI Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

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All lanes : Anti-PECI Antibody (C-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PECI Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
PECI Antibody (C-term) (Cat. #AW5533) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, IHC-P, WB
Primary Accession	O75521
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	43585 Da
Isotype	Rabbit IgG
Antigen Source	HUMAN

Additional Information
Gene ID	10455
Antigen Region	331-358 aa
Other Names	Enoyl-CoA delta isomerase 2, mitochondrial, DRS-1, Delta(3), delta(2)-enoyl-CoA isomerase, D3, D2-enoyl-CoA isomerase, Diazepam-binding inhibitor-related protein 1, DBI-related protein 1, Dodecenoyl-CoA isomerase, Hepatocellular carcinoma-associated antigen 88, Peroxisomal 3, 2-trans-enoyl-CoA isomerase, pECI, Renal carcinoma antigen NY-REN-1, ECI2, DRS1, HCA88, PECI
Dilution	FC~~1:10~50 IHC-P~~1:100~500 WB~~1:1000
Target/Specificity	This PECI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-358 amino acids from the C-terminal region of human PECI.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	PECI Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	ECI2
Synonyms	DRS1, HCA88, PECI {ECO:0000303\|PubMed:10
Function	Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates.
Cellular Location	[Isoform 1]: Mitochondrion {ECO:0000250\|UniProtKB:Q5XIC0}
Tissue Location	Abundant in heart, skeletal muscle and liver. Expressed in CD34(+) T-cells and CD34(+) bone marrow cells

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.