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Anti-PECI Antibody

Western blot analysis of PECI expression in HEK293T (A), Hela (B) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 40 kD)
Immunohistochemical analysis of PECI staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of PECI staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	O75521
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	43585 Da

Additional Information
Gene ID	10455
Other Names	DRS1; HCA88; PECI; Enoyl-CoA delta isomerase 2 mitochondrial; DRS-1; Delta(3), delta(2)-enoyl-CoA isomerase; D3, D2-enoyl-CoA isomerase; Diazepam-binding inhibitor-related protein 1; DBI-related protein 1; Dodecenoyl-CoA isomerase; Hepatocellular carcinoma-associated antigen 88; Peroxisomal 3, 2-trans-enoyl-CoA isomerase; pECI; Renal carcinoma antigen NY-REN-1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PECI. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ECI2
Synonyms	DRS1, HCA88, PECI {ECO:0000303\|PubMed:10
Function	Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates.
Cellular Location	[Isoform 1]: Mitochondrion {ECO:0000250\|UniProtKB:Q5XIC0}
Tissue Location	Abundant in heart, skeletal muscle and liver. Expressed in CD34(+) T-cells and CD34(+) bone marrow cells

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Suk K.,et al.Biochim. Biophys. Acta 1454:126-131(1999).
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mungall A.J.,et al.Nature 425:805-811(2003).
Geisbrecht B.V.,et al.J. Biol. Chem. 274:21797-21803(1999).

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¥ 1,250.00

Cat# AP51175

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Anti-PECI Antibody

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