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PCBP1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - PCBP1 Antibody (Center) AW5581
    All lanes : Anti-PCBP1 Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: 293T/17 whole cell lysate Lane 5: mouse skeletal muscle lysate Lane 6: mouse spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q15365
Other Accession Q5E9A3, P60335, O19048
Reactivity Human
Predicted Human, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 37498 Da
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 5093
Antigen Region 188-217 aa
Other Names Poly(rC)-binding protein 1, Alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, hnRNP E1, Nucleic acid-binding protein SUB23, PCBP1
Dilution WB~~1:2000
Target/Specificity This PCBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-217 amino acids from the Central region of human PCBP1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPCBP1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PCBP1 {ECO:0000303|PubMed:7607214, ECO:0000312|HGNC:HGNC:8647}
Function Single-stranded nucleic acid binding protein that binds preferentially to oligo dC (PubMed:15731341, PubMed:7556077, PubMed:7607214, PubMed:8152927). Together with PCBP2, required for erythropoiesis, possibly by regulating mRNA splicing (By similarity).
Cellular Location Nucleus. Cytoplasm. Note=Loosely bound in the nucleus (PubMed:7607214). May shuttle between the nucleus and the cytoplasm (PubMed:7607214).
Tissue Location Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.

REFERENCES

Cloke, B., et al. Endocrinology 151(8):3954-3964(2010)
Wang, H., et al. Cancer Cell 18(1):52-62(2010)
Zhang, T., et al. Mol. Cancer 9, 72 (2010) :
Waggoner, S.A., et al. J. Biol. Chem. 284(14):9039-9049(2009)
Huo, L.R., et al. Biochim. Biophys. Acta 1784(11):1524-1533(2008)

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