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GMCL1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

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All lanes : Anti-GMCL1 Antibody (Center) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: 293T/17 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: mouse testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AW5625 staining GMCL1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC, WB
Primary Accession	Q96IK5
Other Accession	Q8NEA9
Reactivity	Human, Mouse
Predicted	Monkey
Host	Rabbit
Clonality	Polyclonal
Calculated MW	H=59,H=60[Q8NEA9];M=60 KDa
Isotype	Rabbit Ig
Antigen Source	HUMAN

Additional Information
Gene ID	64395
Antigen Region	281-315
Other Names	Germ cell-less protein-like 1, GMCL1, GCL
Dilution	WB~~0.25 IHC~~1:25
Target/Specificity	This GMCL1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 281-315 amino acids from the Central region of human GMCL1.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GMCL1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	GMCL1
Synonyms	GCL
Function	Possible function in spermatogenesis. Enhances the degradation of MDM2 and increases the amount of p53 probably by modulating the nucleocytoplasmic transport (By similarity).
Cellular Location	Nucleus matrix.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Possible function in spermatogenesis. Enhances the degradation of MDM2 and increases the amount of p53 probably by modulating the nucleocytoplasmic transport (By similarity).

REFERENCES

Ota T.,et al.Nat. Genet. 36:40-45(2004).
Holaska J.M.,et al.J. Biol. Chem. 278:6969-6975(2003).

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Cat# AW5607

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GMCL1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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