PPP2R1B Antibody

Purified Mouse Monoclonal Antibody (Mab)

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All lanes : Anti-PPP2R1B Antibody at 1:2000 dilution Lane 1: human brain lysate Lane 2: Jurkat whole cell lysate Lane 3: mouse brain lysate Lane 4: mouse lung lysate Lane 5: rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing Jurkat cells stained with AW5608 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AW5608, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
AW5608 staining PPP2R1B in human spleen sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma cell line) cells labeling Pdx1 with AW5608 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

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背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, FC
Primary Accession	P30154
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Calculated MW	66214 Da
Isotype	IgG1,Κ
Antigen Source	HUMAN

Additional Information
Gene ID	5519
Antigen Region	53-215 aa
Other Names	Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform, PP2A subunit A isoform PR65-beta, PP2A subunit A isoform R1-beta, PPP2R1B
Dilution	WB~~ 1:2000 IHC~~1:100~500 IF~~1:25 FC~~1:25
Target/Specificity	This PPP2R1B antibody is generated from a mouse immunized with a recombinant protein of human PPP2R1B.
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	PPP2R1B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	PPP2R1B
Function	The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.

REFERENCES

Baysal B.E.,et al.Gene 217:107-116(1998).
Wang S.S.,et al.Science 282:284-287(1998).
Baysal B.E.,et al.Eur. J. Hum. Genet. 9:121-129(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Taylor T.D.,et al.Nature 440:497-500(2006).

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¥ 2,600.00

Cat# AW5610

Size:

100 µl

Price:

¥ 2,600.00

Quantity:

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Availability: In Stock

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PPP2R1B Antibody

Purified Mouse Monoclonal Antibody (Mab)

BACKGROUND

REFERENCES

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