CD27 Ligand/CD70

Product Information
Primary Accession	P32970
Species	Human
Sequence	Gln39-Pro193
Purity	> 95% as analyzed by SDS-PAGE
Endotoxin Level	< 1 EU/ µg of protein by gel clotting method
Biological Activity	Immobilized CD27 (Mammalian, C-Fc-6His) at 1.0 µg/ml (100 µl/well) can bind CD27 Ligand/CD70, hFc, Human with EC₅₀=18.33 ng/ml when detected by SA-HRP.
Expression System	HEK 293
Formulation	Lyophilized from a 0.2 µm filtered solution in PBS.
Reconstitution	It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 µg/ml.
Storage & Stability	Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Additional Information
Gene ID	970
Other Names	CD70 antigen, CD27 ligand, CD27-L, Tumor necrosis factor ligand superfamily member 7 {ECO:0000312\|HGNC:HGNC:11937}, CD70, CD70 (HGNC:11937)
Target Background	CD70 (Cluster of Differentiation 70) is a protein that in humans is encoded by CD70 gene. CD70 is a ligand for CD27. The CD70-CD27 pathway plays an important role in the generation and maintenance of T cell immunity, in particular during antiviral responses. Upon CD27 binding, induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.The CD70 protein is expressed on highly activated lymphocytes (like in T- and B-cell lymphomas). It is therefore suggested that anti-CD70 antibodies might be a possible treatment for CD70 positive lymphomas as normal lymphocytes have low CD70 expression.

Protein Information
Name	CD70 (HGNC:11937)
Function	Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells (PubMed:28011863, PubMed:28011864, PubMed:8387892). The CD70- CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells (PubMed:28011863, PubMed:28011864).
Cellular Location	Cell membrane; Single-pass type II membrane protein