癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
VISTA/B7-H5
- 产品详情
- 实验流程
| Primary Accession | Q9H7M9 |
|---|---|
| Species | Human |
| Sequence | Phe33-Ala194 |
| Purity | > 90% as analyzed by SDS-PAGE |
| Endotoxin Level | < 1 EU/ µg of protein by gel clotting method |
| Biological Activity | Immobilized Human IGSF11 at 2.0 µg/ml (100 µl/well) can bind VISTA/B7-H5, hFc, Human with a linear range of 1.85-50.0 µg/ml when detected by SA-HRP. |
| Expression System | HEK 293 |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS. |
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 µg/ml. |
| Storage & Stability | Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Gene ID | 64115 |
|---|---|
| Other Names | V-type immunoglobulin domain-containing suppressor of T-cell activation, Platelet receptor Gi24 {ECO:0000303|Ref.1}, Stress-induced secreted protein-1 {ECO:0000303|Ref.2}, Sisp-1 {ECO:0000303|Ref.2}, V-set domain-containing immunoregulatory receptor, VSIR (HGNC:30085) |
| Target Background | V-domain Ig suppressor of T cell activation (VISTA), also known as B7-H5, is a type I transmembrane protein that functions as an immune checkpoint. VISTA belongs to the immunoglobulin superfamily and has one IgV domain. It is primarily expressed in white blood cells and its transcription is partially controlled by p53. VISTA can act as both a ligand and a receptor on T cells to inhibit T cell effector function and maintain peripheral tolerance. VISTA may also promote differentiation of embryonic stem cells by inhibiting BMP4 signaling (By similarity) and may stimulate MMP14-mediated MMP2 activation. |
| Name | VSIR (HGNC:30085) |
|---|---|
| Function | Immunoregulatory receptor which inhibits the T-cell response (PubMed:24691993). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (By similarity). May stimulate MMP14- mediated MMP2 activation (PubMed:20666777). |
| Cellular Location | Cell membrane; Single-pass type I membrane protein |
| Tissue Location | Expressed in spleen. Detected on a number of myeloid cells including CD11b monocytes, CD66b+ neutrophils, at low levels on CD4+ and CD8+ T-cells, and in a subset of NK cells. Not detected on B cells (at protein level). Expressed at high levels in placenta, spleen, plasma blood leukocytes, and lung. Expressed at moderate levels in lymph node, bone marrow, fat, uterus, and trachea Has low expression levels in other tissues |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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