LEF-1

Rabbit Monoclonal antibody(Mab)

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P
Primary Accession	Q9UJU2
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Clone Names	892B2A3
Calculated MW	44201 Da

Additional Information
Gene ID	51176
Gene Name	LEF1
Other Names	Lymphoid enhancer-binding factor 1, LEF-1, T cell-specific transcription factor 1-alpha, TCF1-alpha, LEF1
Dilution	IHC-P~~Ready-to-use
Storage	Maintain refrigerated at 2-8°C.
Precautions	LEF-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	LEF1 (HGNC:6551)
Function	Transcription factor that binds DNA in a sequence-specific manner (PubMed:2010090). Participates in the Wnt signaling pathway (By similarity). Activates transcription of target genes in the presence of CTNNB1 and EP300 (By similarity). PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1 (PubMed:11266540). Regulates T-cell receptor alpha enhancer function (PubMed:19653274). Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling (By similarity). May play a role in hair cell differentiation and follicle morphogenesis (By similarity).
Cellular Location	Nucleus {ECO:0000255\|PROSITE-ProRule:PRU00267}. Note=Found in nuclear bodies upon PIASG binding.
Tissue Location	Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.