Goat anti-CTGF Antibody

Peptide-affinity purified goat antibody

EB11760 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB11760 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB11760 (6µg/ml) staining of paraffin embedded Rat Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB11760 Negative Control showing staining of paraffin embedded Rat Liver, with no primary antibody.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IF, FC, Pep-ELISA
Primary Accession	P29279
Other Accession	NP_001892.1
Reactivity	Human, Mouse, Rat, Pig, Dog, Bovine
Host	Goat
Clonality	Polyclonal
Clone Names	CCN2
Calculated MW	38091 Da

Additional Information
Gene ID	1490
Other Names	CTGF; connective tissue growth factor; CCN2; HCS24; IGFBP8; MGC102839; NOV2; CCN family member 2; IBP-8; IGF-binding protein 8; IGFBP-8; OTTHUMP00000017213; hypertrophic chondrocyte-specific protein 24; insulin-like growth factor-binding protein 8
Dilution	IF~~1:50~200 FC~~1:10~50 Pep-ELISA~~N/A
Format	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Immunogen	Immunizing peptide overlaps disulphide bond
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	Goat anti-CTGF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CCN2 (HGNC:2500)
Function	Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Is involved in the stimulation of osteoblast differentiation and has a critical role in osteogenesis (PubMed:39414788). Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor- induced DNA synthesis.
Cellular Location	Secreted, extracellular space, extracellular matrix {ECO:0000250\|UniProtKB:P29268}. Secreted
Tissue Location	Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.