CD1A Antibody

Purified Mouse Monoclonal Antibody

Red: Control Antigen (100ng);
Purple: Antigen (10ng);
Green: Antigen (50ng);
Blue: Antigen (100ng);
Figure 1: Western blot analysis using CD1A mouse mAb against K562 (1), RAJI (2), and MOLT4 (3) cell lysate.
Figure 2: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues (left) and colon cancer tissues (right) using CD1A mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded brain tissues (left) and submaxillary tumor tissues (right) using CD1A mouse mAb with DAB staining.
Figure 4: Immunofluorescence analysis of RAJI cells using CD1A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P06126
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	7A7
Isotype	IgG1
Calculated MW	37077 Da
Description	CD1a is a non polymorphic MHC Class 1 related cell surface glycoprotein, expressed in association with Beta 2 microglobulin. CD1a is expressed by cortical thymocytes, Langerhan's cells and by interdigitating cells. CD1a is also expressed by some malignancies of T cell lineage and in histiocytosis X. Tissue specificity: Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues.
Immunogen	Purified recombinant fragment of human CD1A expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Gene ID	909
Other Names	T-cell surface glycoprotein CD1a, T-cell surface antigen T6/Leu-6, hTa1 thymocyte antigen, CD1a, CD1A
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CD1A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CD1A
Function	Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
Cellular Location	Cell membrane; Single-pass type I membrane protein. Membrane raft; Single-pass type I membrane protein. Endosome membrane; Single- pass type I membrane protein. Note=Subject to intracellular trafficking between the cell membrane and endosomes (PubMed:11231314). Localizes to cell surface lipid rafts (PubMed:18178838).
Tissue Location	Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.