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CD30 Antibody

Purified Mouse Monoclonal Antibody

     
  • 10 - CD30 Antibody AO1674a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • 1 - CD30 Antibody AO1674a
    Figure 1: Western blot analysis using CD30 mAb against human CD30 (AA: 536-590) recombinant protein. (Expected MW is 32 kDa)
  • 1 - CD30 Antibody AO1674a
    Figure 2: Western blot analysis using CD30 mouse mAb against Hela cell lysate.
  • 4 - CD30 Antibody AO1674a
    Figure 3: Flow cytometric analysis of Hela cells using CD30 mouse mAb (green) and negative control (red).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession P28908
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3B10
Isotype IgG1
Calculated MW 63747 Da
Description The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. 
Immunogen Purified recombinant fragment of human CD30 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 943
Other Names Tumor necrosis factor receptor superfamily member 8, CD30L receptor, Ki-1 antigen, Lymphocyte activation antigen CD30, CD30, TNFRSF8, CD30, D1S166E
Dilution WB~~1/500 - 1/2000
FC~~1/200 - 1/400
E~~1/10000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD30 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TNFRSF8 (HGNC:11923)
Function Receptor for TNFSF8/CD30L (PubMed:8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa- B (PubMed:8999898).
Cellular Location [Isoform 1]: Cell membrane; Single-pass type I membrane protein
Tissue Location [Isoform 2]: Detected in alveolar macrophages (at protein level).
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

REFERENCES

1. Exp Clin Transplant. 2009 Dec;7(4):237-40. 2. Mol Vis. 2009 Oct 17;15:2068-73.

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