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LEF1 Antibody

Purified Mouse Monoclonal Antibody

     
  • 10 - LEF1 Antibody AO2191a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • 1 - LEF1 Antibody AO2191a

    Figure 2:Western blot analysis using LEF1 mAb against human LEF1 (AA: 33-138) recombinant protein. (Expected MW is 37.1 kDa)

  • 1 - LEF1 Antibody AO2191a

    Figure 3:Western blot analysis using LEF1 mAb against HEK293 (1) and LEF1 (AA: 33-138)-hIgGFc transfected HEK293 (2) cell lysate.

  • 2 - LEF1 Antibody AO2191a

    Figure 4:Immunohistochemical analysis of paraffin-embedded HeLa tissues using LEF1 mouse mAb with DAB staining.

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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, E
Primary Accession Q9UJU2
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3G5F9
Isotype IgG1
Calculated MW 44201 Da
Description This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.
Immunogen Purified recombinant fragment of human LEF1 (AA: 33-138) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 51176
Other Names Lymphoid enhancer-binding factor 1, LEF-1, T cell-specific transcription factor 1-alpha, TCF1-alpha, LEF1
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
E~~1/10000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLEF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LEF1 (HGNC:6551)
Function Transcription factor that binds DNA in a sequence-specific manner (PubMed:2010090). Participates in the Wnt signaling pathway (By similarity). Activates transcription of target genes in the presence of CTNNB1 and EP300 (By similarity). PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1 (PubMed:11266540). Regulates T-cell receptor alpha enhancer function (PubMed:19653274). Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling (By similarity). May play a role in hair cell differentiation and follicle morphogenesis (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00267}. Note=Found in nuclear bodies upon PIASG binding.
Tissue Location Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

REFERENCES

1.Cancer Invest. 2014 Aug;32(7):368-74.2.BMC Gastroenterol. 2012 May 28;12:53.

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