ALPI Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using ALPI mAb against human ALPI recombinant protein. (Expected MW is 31.9 kDa)
Figure 2: Western blot analysis using ALPI mAb against HEK293 (1) and ALPI (AA: 397-458)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Western blot analysis using ALPI mouse mAb against HL60 (1) and HepG2 (2) cell lysate.
Figure 5: Flow cytometric analysis of HepG2 cells using ALPI mouse mAb (green) and negative control (purple).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	P09923
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	3B10E7
Isotype	IgG1
Calculated MW	56812 Da
Description	There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.
Immunogen	Purified recombinant fragment of human ALPI (AA: 397-458) expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Gene ID	248
Other Names	Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, 3.1.3.1, ALPI
Dilution	WB~~1/500 - 1/2000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ALPI Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	ALPI
Function	Alkaline phosphatase that can hydrolyze various phosphate compounds.
Cellular Location	Cell membrane {ECO:0000250\|UniProtKB:P15693}; Lipid-anchor, GPI-anchor {ECO:0000250\|UniProtKB:P15693}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.