SKOR2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of lysates from human cerebellum, human testis, mouse cerebellum tissue lysate (from left to right), using SKOR2 Antibody (C-term)(Cat. #AP21005c). AP21005c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
AP21005c staining SKOR2 in mouse cerebellum at embryonic stage 12.5 sections by Immunohistochemistry (IHC-P - 4%paraformaldehyde-fixed, cryopreserved in 30% sucrose/1X PBS for 30 hours, and then cut at a cryotome). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature. Samples were incubated with primary antibody (1/2000) for 1 hours at 37°C.Immunofluorescence image showing purkinje cell neurons staining on mouse cerebellum tissue.The nuclear counter stain is DAPI (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF, E
Primary Accession	Q2VWA4
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	105809 Da

Additional Information
Gene ID	652991
Other Names	SKI family transcriptional corepressor 2, Functional Smad-suppressing element on chromosome 18, Fussel-18, LBX1 corepressor 1-like protein, Ladybird homeobox corepressor 1-like protein, SKOR2, CORL2, FUSSEL18
Target/Specificity	This SKOR2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 741-775 amino acids from the C-terminal region of human SKOR2.
Dilution	WB~~1:1000 IF~~1:2000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	SKOR2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	SKOR2 (HGNC:32695)
Synonyms	CORL2, FUSSEL18
Function	Exhibits transcriptional repressor activity (By similarity). Acts as a TGF-beta antagonist in the nervous system.
Cellular Location	Nucleus. Cytoplasm
Tissue Location	Expressed in cerebellum, spinal cord and testis. Isoform 2 is present in cerebellum (at protein level)

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.