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Anti-PPP2R1B Antibody

Western blot analysis of PPP2R1B expression in HEK293T (A), Hela (B), HGC27 (C), mouse testis (D), rat testis (E) whole cell lysates. (Predicted band size: 66 kD; Observed band size: 66 kD)
Immunohistochemical analysis of PPP2R1B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of PPP2R1B staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P30154
Reactivity	Human, Mouse, Rat, Pig, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	66214 Da

Additional Information
Gene ID	5519
Other Names	Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform; PP2A subunit A isoform PR65-beta; PP2A subunit A isoform R1-beta
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PPP2R1B. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	PPP2R1B
Function	The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Baysal B.E.,et al.Gene 217:107-116(1998).
Wang S.S.,et al.Science 282:284-287(1998).
Baysal B.E.,et al.Eur. J. Hum. Genet. 9:121-129(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Taylor T.D.,et al.Nature 440:497-500(2006).

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¥ 1,250.00

Cat# AP51856

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Anti-PPP2R1B Antibody

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