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>   首页   >   产品   >   一抗   >   癌症   >   Rabbit Anti-CYP11A1 Polyclonal Antibody   

Rabbit Anti-CYP11A1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Rabbit Anti-CYP11A1 Polyclonal Antibody AP52081
    Lane 1: mouse kidney lysates Lane 2: human kidney lysates probed with Anti CYP11A1/P450SCC Polyclonal Antibody, Unconjugated (AP52081) at 1:200 in 4˚C. Followed by conjugation to secondary antibody at 1:3000 90min in 37˚C. Predicted band 53/57kD. Observed band size: 53kD.
  • 14 - Rabbit Anti-CYP11A1 Polyclonal Antibody AP52081
    Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti CYP11A1/P450SCC Polyclonal Antibody, Unconjugated (AP52081) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
  • 1 - Rabbit Anti-CYP11A1 Polyclonal Antibody AP52081
    Mouse placenta lysates probed with Rabbit Anti-CYP11A1 Polyclonal Antibody, Unconjugated (AP52081) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:500 for 90 min at 37˚C.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IHC-F, IF
Primary Accession P05108
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 60102 Da
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human CYP11A1/P450SCC
Epitope Specificity 321-420/521
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Mitochondrion membrane.
SIMILARITY Belongs to the cytochrome P450 family.
DISEASE Defects in CYP11A1 are the cause of adrenal insufficiency congenital with 46,XY sex reversal (AICSR) [MIM:613743]. A rare disorder that can present as acute adrenal insufficiency in infancy or childhood. ACTH and plasma renin activity are elevated and adrenal steroids are inappropriately low or absent; the 46,XY patients have female external genitalia, sometimes with clitoromegaly. The phenotypic spectrum ranges from prematurity, complete underandrogenization, and severe early-onset adrenal failure to term birth with clitoromegaly and later-onset adrenal failure. Patients with congenital adrenal insufficiency do not manifest the massive adrenal enlargement typical of congenital lipoid adrenal hyperplasia.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]
Additional Information
Gene ID 1583
Other Names CYP11A; CYPXIA1; P45SCC; Cholesterol side-chain cleavage enzyme, mitochondrial; Cholesterol desmolase; Cytochrome P45 11A1; Cytochrome P45(scc); CYP11A1
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Format0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name CYP11A1 {ECO:0000303|PubMed:21636783, ECO:0000312|HGNC:HGNC:2590}
Function A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones (PubMed:21636783). Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R- hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal (PubMed:21636783). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin) (PubMed:21636783).
Cellular Location Mitochondrion inner membrane {ECO:0000250|UniProtKB:P14137}; Peripheral membrane protein. Note=Localizes to the matrix side of the mitochondrion inner membrane. {ECO:0000250|UniProtKB:P14137}
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.

REFERENCES

Chung B.-C.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:8962-8966(1986).
Morohashi K.,et al.J. Biochem. 101:879-887(1987).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Zody M.C.,et al.Nature 440:671-675(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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