Anti-Cytochrome P450 11A1 Antibody

Western blot analysis of Cytochrome P450 11A1 expression in HEK293T (A), Hela (B), H460 (C), mouse lung (D) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 60 kD)
Immunohistochemical analysis of Cytochrome P450 11A1 staining in rat heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Cytochrome P450 11A1 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P05108
Reactivity	Human, Mouse, Rat, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	60102 Da

Additional Information
Gene ID	1583
Other Names	CYP11A; Cholesterol side-chain cleavage enzyme mitochondrial; CYPXIA1; Cholesterol desmolase; Cytochrome P450 11A1; Cytochrome P450(scc)
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cytochrome P450 11A1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CYP11A1 {ECO:0000303\|PubMed:21636783, ECO:0000312\|HGNC:HGNC:2590}
Function	A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones (PubMed:21636783). Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R- hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal (PubMed:21636783). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin) (PubMed:21636783).
Cellular Location	Mitochondrion inner membrane {ECO:0000250\|UniProtKB:P14137}; Peripheral membrane protein. Note=Localizes to the matrix side of the mitochondrion inner membrane. {ECO:0000250\|UniProtKB:P14137}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.