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>   首页   >   产品   >   一抗   >   其他   >   Anti-Kalirin Antibody   

Anti-Kalirin Antibody

     
  • 1 - Anti-Kalirin Antibody AP53717
    Western blot analysis of Kalirin expression in Hela (A), H446 (B) whole cell lysates.
  • 3 - Anti-Kalirin Antibody AP53717
    Immunofluorescent analysis of Kalirin staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF
Primary Accession O60229
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 340261 Da
Additional Information
Gene ID 8997
Other Names DUET; DUO; HAPIP; TRAD; Kalirin; Huntingtin-associated protein-interacting protein; Protein Duo; Serine/threonine-protein kinase with Dbl- and pleckstrin homology domain
Target/Specificity Recognizes endogenous levels of Kalirin protein.
Dilution WB~~1/500 - 1/1000
IF~~1/50 - 1/200
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name KALRN (HGNC:4814)
Synonyms DUET, DUO, HAPIP, TRAD
Function Promotes the exchange of GDP by GTP. Activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. Induces lamellipodia independent of its GEF activity.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Note=Associated with the cytoskeleton
Tissue Location Isoform 2 is brain specific. Highly expressed in cerebral cortex, putamen, amygdala, hippocampus and caudate nucleus Weakly expressed in brain stem and cerebellum. Isoform 4 is expressed in skeletal muscle.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Rabbit polyclonal antibody to Kalirin

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