Anti-Actin-pan (Acetyl-K52/50/51) Antibody

Western blot analysis of Actin-pan (Acetyl-K52/50/51) expression in mouse muscle (A) whole cell lysates. (Predicted band size: 41; 42 kD; Observed band size: 42 kD)
Immunofluorescent analysis of Actin-pan (Acetyl-K52/50/51) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P62736
Other Accession	P60709, P68032, P63261, P63267, P68133
Reactivity	Human, Mouse, Rat, Chicken, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	42009 Da

Additional Information
Gene ID	59
Other Names	ACTA2; ACTSA; ACTVS; GIG46; Actin aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein; ACTB; Actin cytoplasmic 1; Beta-actin; ACTC1; ACTC; Actin alpha cardiac muscle 1; Alpha-cardiac actin; ACTG1; ACTB; ACTG; Actin cytoplasmic 2; Gamma-actin; ACTG2; ACTA3; ACTL3; ACTSG; Actin gamma-enteric smooth muscle; Alpha-actin-3; Gamma-2-actin; Smooth muscle gamma-actin; ACTA1; ACTA; Actin alpha skeletal muscle; Alpha-actin-1
Target/Specificity	KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K52/50/51 of human Actin-pan protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ACTA2
Synonyms	ACTSA, ACTVS
Function	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location	Cytoplasm, cytoskeleton.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.