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NMES1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - NMES1 Polyclonal Antibody AP57465
    Paraformaldehyde-fixed, paraffin embedded (Rat intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NMES1) Polyclonal Antibody, Unconjugated (AP57465) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IHC-F, IF, ICC, E
Primary Accession Q9C002
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 9617 Da
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human NMES1
Epitope Specificity 31-83/83
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Nucleus.
SIMILARITY Belongs to the complex I NDUFA4 subunit family.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions This gene was first identified in a study of human esophageal squamous cell carcinoma tissues. Levels of both the message and protein are reduced in carcinoma samples. In adult human tissues, this gene is expressed in the the esophagus, stomach, small intestine, colon and placenta. Alternatively spliced transcript variants that encode the same protein have been identified. [provided by RefSeq, Jun 2012]
Additional Information
Gene ID 84419
Other Names Normal mucosa of esophagus-specific gene 1 protein, Protein FOAP-11, NMES1, C15orf48
Target/Specificity Expressed mainly in stomach, placenta, small intestine and colon, as well as in normal mucosa of esophagus. Down-regulated in esophageal squamous cell carcinoma.
Dilution IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name NMES1
Synonyms C15orf48
Cellular Location Nucleus.
Tissue Location Expressed mainly in stomach, placenta, small intestine and colon, as well as in normal mucosa of esophagus. Down- regulated in esophageal squamous cell carcinoma
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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