Anti-CLIP2 Antibody

Western blot analysis of CLIP2 expression in AML12 (A), PC12 (B), A549 (C), LO2 (D), Hela (E) whole cell lysates. (Predicted band size: 115 kD; Observed band size: 130 kD)
Immunofluorescent analysis of Vimentin staining in CLIP2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q9UDT6
Reactivity	Human, Mouse, Rat, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	115837 Da

Additional Information
Gene ID	7461
Other Names	CYLN2; KIAA0291; WBSCR3; WBSCR4; WSCR4; CAP-Gly domain-containing linker protein 2; Cytoplasmic linker protein 115; CLIP-115; Cytoplasmic linker protein 2; Williams-Beuren syndrome chromosomal region 3 protein; Williams-Beuren syndrome chromosomal region 4 protein
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CLIP2. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CLIP2
Synonyms	CYLN2, KIAA0291, WBSCR3, WBSCR4, WSCR4
Function	Seems to link microtubules to dendritic lamellar body (DLB), a membranous organelle predominantly present in bulbous dendritic appendages of neurons linked by dendrodendritic gap junctions. May operate in the control of brain-specific organelle translocations (By similarity).
Cellular Location	Cytoplasm {ECO:0000250\|UniProtKB:O55156}. Cytoplasm, cytoskeleton {ECO:0000250\|UniProtKB:Q9Z0H8}. Note=Localizes preferentially to the ends of tyrosinated microtubules {ECO:0000250\|UniProtKB:Q9Z0H8}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.