Anti-Rabaptin 5 beta Antibody

Rabbit polyclonal antibody to Rabaptin 5 beta

Western blot analysis of Rabaptin 5 beta expression in BV2 (A), PC12 (B), A549 (C), LOVO (D) whole cell lysates.
Immunohistochemical analysis of Rabaptin 5 beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IP, IHC
Primary Accession	Q9H5N1
Reactivity	Human, Mouse, Rat, Monkey
Host	Rabbit
Clonality	Polyclonal
Calculated MW	63543 Da

Additional Information
Gene ID	79874
Other Names	RABPT5B; Rab GTPase-binding effector protein 2; Rabaptin-5beta
Target/Specificity	Recognizes endogenous levels of Rabaptin 5 beta protein.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IP (1/10 - 1/100) IP~~N/A IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IP (1/10 - 1/100)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	RABEP2
Synonyms	RABPT5B
Function	Plays a role in membrane trafficking and in homotypic early endosome fusion (PubMed:9524116). Participates in arteriogenesis by regulating vascular endothelial growth factor receptor 2/VEGFR2 cell surface expression and endosomal trafficking (PubMed:29425100). By interacting with SDCCAG8, localizes to centrosomes and plays a critical role in ciliogenesis (PubMed:27224062).
Cellular Location	Cytoplasm. Early endosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, cilium basal body

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.