Anti-JIP4 Antibody

Western blot analysis of JIP4 expression in AML12 (A), U87MG (B), EC9706 (C), SGC7901 (D) whole cell lysates. (Predicted band size: 146 kD; Observed band size: 190 kD)
Immunofluorescent analysis of JIP4 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	O60271
Reactivity	Human, Mouse, Rat, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	146205 Da

Additional Information
Gene ID	9043
Other Names	HSS; KIAA0516; MAPK8IP4; SYD1; C-Jun-amino-terminal kinase-interacting protein 4; JIP-4; JNK-interacting protein 4; Cancer/testis antigen 89; CT89; Human lung cancer oncogene 6 protein; HLC-6; JNK-associated leucine-zipper protein; JLP; Mitogen-activated protein kinase 8-interacting protein 4; Proliferation-inducing protein 6; Protein highly expressed in testis; PHET; Sperm surface protein; Sperm-associated antigen 9; Sperm-specific protein; Sunday driver 1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human JIP4. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SPAG9 (HGNC:14524)
Function	The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module (PubMed:14743216). Regulates lysosomal positioning by acting as an adapter protein which links PIP4P1-positive lysosomes to the dynein- dynactin complex (PubMed:29146937). Assists PIKFYVE selective functionality in microtubule-based endosome-to-TGN trafficking (By similarity).
Cellular Location	Cytoplasm {ECO:0000250\|UniProtKB:Q58A65}. Cytoplasm, perinuclear region {ECO:0000250\|UniProtKB:Q58A65}. Lysosome membrane. Note=Perinuclear distribution in response to stress signals such as UV radiation {ECO:0000250\|UniProtKB:Q58A65}
Tissue Location	Expressed only in testis on the round spermatids of stage I, II and II. Absent in spermatogonia and spermatocyte [Isoform 3]: Expressed in testis.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.