|Application ||IHC-P-Leica, WB, E|
|Calculated MW||146205 Da|
|Other Names||C-Jun-amino-terminal kinase-interacting protein 4, JIP-4, JNK-interacting protein 4, Cancer/testis antigen 89, CT89, Human lung cancer oncogene 6 protein, HLC-6, JNK-associated leucine-zipper protein, JLP, Mitogen-activated protein kinase 8-interacting protein 4, Proliferation-inducing protein 6, Protein highly expressed in testis, PHET, Sperm surface protein, Sperm-associated antigen 9, Sperm-specific protein, Sunday driver 1, SPAG9, HSS, KIAA0516, MAPK8IP4, SYD1|
|Target/Specificity||This JLP (SPAG9) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-120 amino acids from the N-terminal region of human JLP (SPAG9).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||JLP (SPAG9) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||HSS, KIAA0516, MAPK8IP4, SYD1|
|Function||The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Isoform 5 may play a role in spermatozoa-egg- interaction.|
|Cellular Location||Cytoplasm. Cytoplasm, perinuclear region. Note=Perinuclear distribution in response to stress signals such as UV radiation|
|Tissue Location||Isoform 5 is expressed only in testis on the round spermatids of stage I, II and II. Isoform 5 is absent in spermatogonia and spermatocyte. Isoform 3 is expressed in testis Isoform 4 is expressed in testis and in acute myeloid leukemia (AML) patients.|
Provided below are standard protocols that you may find useful for product applications.
SPAG9, which is abundantly expressed in testicular haploid germ cells, is recognized by sperm-agglutinating antibodies and implicated in infertility.
Rana,R., Hum. Reprod. 21 (11), 2894-2900 (2006)
Rana,R., Biochem. Biophys. Res. Commun. 340 (1), 158-164 (2006)
Jagadish,N., Keio J Med 54 (2), 66-71 (2005)