Anti-ZEB2 Antibody

Rabbit polyclonal antibody to ZEB2

Western blot analysis of ZEB2 expression in mouse liver (A) whole cell lysates.
Immunohistochemical analysis of ZEB2 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	O60315
Reactivity	Human, Rat, Rabbit, Monkey
Host	Rabbit
Clonality	Polyclonal
Calculated MW	136447 Da

Additional Information
Gene ID	9839
Other Names	KIAA0569; SIP1; ZFHX1B; ZFX1B; Zinc finger E-box-binding homeobox 2; Smad-interacting protein 1; SMADIP1; Zinc finger homeobox protein 1b
Target/Specificity	Recognizes endogenous levels of ZEB2 protein.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200) IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	ZEB2 (HGNC:14881)
Function	Transcriptional inhibitor that binds to DNA sequence 5'- CACCT-3' in different promoters (PubMed:16061479, PubMed:20516212). Represses transcription of E-cadherin (PubMed:16061479). Represses expression of MEOX2 (PubMed:20516212).
Cellular Location	Nucleus. Chromosome